Difference between revisions of "Part:BBa K3725210"

Revision as of 16:46, 20 October 2021

Phosphorylated NarL-Specific Promoter Sequence (pNar)

pNar is a regulatory promoter responsive to phosphorylated NarL. This gene is sourced from Bacillus Subtilis; however, it works with the DNA-binding response regulator NarL from the E. coli Nar Operon.

Usage and Biology

Lambert_GA iGEM 2021 developed a cell-free nitrate biosensor, using the pNAR promoter to quantify the amount of nitrate present in a solution. Lambert_GA’s part BBa_K3725220 is a composite part with sfGFP driven by this pNar promoter. The team’s biosensor utilized three plasmids, NarL, NarX and a sfGFP part with the pNAR promoter. This was designed by Dr. Adam Silverman at the Jewett Lab at Northwestern University and shipped to our lab for testing.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

This sequence is only the promoter sequence. For the composite part with sfGFP, visit part BBa_K3725220.

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3725210 short</partinfo>
-pNar is a regulatory promoter responsive to phosphorylated NarL. This gene is modeled after the E.coli natural nitrate sensor, the Nar Operon, which includes the membrane-bound sensor protein NarX and its corresponding DNA-binding response regulator NarL.
+pNar is a regulatory promoter responsive to phosphorylated NarL. This gene is sourced from Bacillus Subtilis; however, it works with the DNA-binding response regulator NarL from the E. coli Nar Operon.
 ===Usage and Biology===
-Lambert_GA iGEM 2021 developed a cell-free nitrate biosensor, using the pNAR promoter to quantify the amount of nitrate present in a solution. Lambert_GA’s part BBa_K3725220 is a composite part with sfGFP driven by this pNar promoter. The team’s biosensor utilized three plasmids, NarL, NarX and a sfGFP part with the pNAR promoter. These plasmids were designed by Dr. Adam Silverman at the Jewett Lab at Northwestern University and shipped to our lab for testing.
+Lambert_GA iGEM 2021 developed a cell-free nitrate biosensor, using the pNAR promoter to quantify the amount of nitrate present in a solution. Lambert_GA’s part BBa_K3725220 is a composite part with sfGFP driven by this pNar promoter. The team’s biosensor utilized three plasmids, NarL, NarX and a sfGFP part with the pNAR promoter. This was designed by Dr. Adam Silverman at the Jewett Lab at Northwestern University and shipped to our lab for testing.
 <span class='h3bb'>Sequence and Features</span>