Difference between revisions of "Part:BBa K3712000"

Revision as of 16:45, 20 October 2021

a kind of elastin like peptide (VPGIG)27

USAGE AND BIOLOGY It is a kind of elastin like peptide we designed. N means the number of VPGIG repeats. ELP (Elastin-like polypeptide) is a thermally responsive polymer that changes phase when it reaches a critical temperature. When the temperature is higher than the critical temperature, ELP will aggregate through hydrophobic action, and the hydrophobic tail will be surrounded in the interior while the hydrophilic head will be exposed. But ELP remains soluble below the critical temperature. ELP is composed of pentapeptide repeat sequence Val-Pro-Gly-Xaa-Gly (Xaa is any amino acid except Pro)[1], and its transition temperature can be controlled by the concentration, the repeat number of ELP repeat sequence and the amino acid type at Xaa. Through theoretical prediction[2], we predict 10mg/L VPGIG27 aggregates at 42℃, which is the temperature used in acne heat treatment, so that heat treatment can combine with our drugs.

RESULTS We use the ELP connected with the Wild-type BLP-7 for ELP functional verification. Under the temperature gradient of 30~50℃, the particle size measurement is carried out for every 1℃ increase. We find that the main peak of ELP aggregates distribution (more than 70%) gradually shifted to the larger particle size as the temperature increased. Location gathering. We averaged the main peaks of the three particle diameter measurements, and then plotted the average value as shown in Figure 1.A. We found that there is an inflection point at 44℃, which means that 44℃ is the transition temperature of Wild-type BLP-7-27 ELP. At the same time, we find that at 44℃ there is a rising inflection point for the Zeta potential, which is caused by rich positive charges of the Wild-type BLP-7 connected with the ELP aggregate.

Sequence and Features