Difference between revisions of "Part:BBa K3725210"

Revision as of 16:26, 20 October 2021

Phosphorylated NarL-Specific Promoter Sequence (pNar)

pNar is a regulatory promoter responsive to phosphorylated NarL. This gene is modeled after the E.coli natural nitrate sensor, the Nar Operon, which includes the membrane-bound sensor protein NarX and its corresponding DNA-binding response regulator NarL.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

This sequence is only the promoter sequence. For the composite part with sfGFP, visit part BBa_K3725220.

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 <partinfo>BBa_K3725210 short</partinfo>
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+pNar is a regulatory promoter responsive to phosphorylated NarL. This gene is modeled after the E.coli natural nitrate sensor, the Nar Operon, which includes the membrane-bound sensor protein NarX and its corresponding DNA-binding response regulator NarL.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+Lambert_GA iGEM 2021 developed a cell-free nitrate biosensor, using the pNAR promoter to quantify the amount of nitrate present in a solution. Lambert_GA’s part BBa_K3725220 is a composite part with sfGFP driven by this pNar promoter. The team’s biosensor utilized three plasmids, NarL, NarX and a sfGFP part with the pNAR promoter. These plasmids were designed by Dr. Adam Silverman at the Jewett Lab at Northwestern University and shipped to our lab for testing.
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 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K3725210 SequenceAndFeatures</partinfo>
+This sequence is only the promoter sequence. For the composite part with sfGFP, visit part BBa_K3725220.
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