Difference between revisions of "Part:BBa K3896017"

Revision as of 16:20, 20 October 2021

Plux_HS-LuxI-EGFP

In the AHL mediated quorum sensing system,after reaching the threshold, AHL can combine with endogenous LuxR to form a complex and activate the corresponding promoter PluxR. In this project, we selected the improved promoter Plux_HS of iGEM2018 Tsinghua.The promoter has the advantage of low leakage.After Plux_ HS is activated, the expressed AHL will combine with endogenous LuxR to form a complex and furtherly activate Plux_ HS promoter, so as to achieve the effect of cascade amplification, and finally express a large number of EGFP, making the detection effect significant.

We first verify the circuits by artificially adding AHL.

We want to know the concentration of AHL and set a series of gradients. The results are as follows.

Fig. tuzhu

Sequence and Features