Difference between revisions of "Part:BBa K592015:Design"

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B0014 instead of B0015 in order to avoid unwanted primer binding, etc. J23101 promoter to ensure strong and constitutive expression at all times.
 
B0014 instead of B0015 in order to avoid unwanted primer binding, etc. J23101 promoter to ensure strong and constitutive expression at all times.
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BBa_K592015 is a amilCP blue coding device, which consists of J23101, B0034, K592009, and B0014. It was constructed by iGEM11_Uppsala-Sweden in Sep. 2011. This device can express blue/purple color protein, which enriches the reporter genes for the research of life science. K592009 is a blue chromo protein gene from the Acropora millepora. AmilCP, naturally exhibits strong color when expressed. The protein has an absorbance maximum at 588 nm giving it a blue/purple color visible to the naked eye, thereby requiring no instruments to observe. The strong color is readily observed in both LB or agar culture, in less than 24 hours of incubation. It is a very useful part.
 
  
 
===Source===
 
===Source===

Revision as of 16:16, 20 October 2021

amilCP Blue Coding Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We found this design very useful when assembling components that contain RFP, mCherry, etc. The assembled parts turn red and are not recognizable from the background insert(BBa_J04450) if all colonies becomes red after transformation. Luckily, very few genes produce things that turn blue/purple, which makes our construct extremely useful.

B0014 instead of B0015 in order to avoid unwanted primer binding, etc. J23101 promoter to ensure strong and constitutive expression at all times.

Source

amilCP comes from the genome of Acropora　millepora. All other parts are assembled from the iGEM 2011 distribution kit.

References