Difference between revisions of "Part:BBa K3896017"
Line 5: Line 5:
 
In the AHL mediated quorum sensing system,after reaching the threshold, AHL can combine with endogenous LuxR to form a complex and activate the corresponding promoter PluxR. In this project, we selected the improved promoter Plux_HS of iGEM2018 Tsinghua.The promoter has the advantage of low leakage.After Plux_ HS is activated, the expressed AHL will combine with endogenous LuxR to form a complex and furtherly activate Plux_ HS promoter, so as to achieve the effect of cascade amplification, and finally express a large number of EGFP, making the detection effect significant.
 
In the AHL mediated quorum sensing system,after reaching the threshold, AHL can combine with endogenous LuxR to form a complex and activate the corresponding promoter PluxR. In this project, we selected the improved promoter Plux_HS of iGEM2018 Tsinghua.The promoter has the advantage of low leakage.After Plux_ HS is activated, the expressed AHL will combine with endogenous LuxR to form a complex and furtherly activate Plux_ HS promoter, so as to achieve the effect of cascade amplification, and finally express a large number of EGFP, making the detection effect significant.
  
−
We first verify the above circuits by artificially adding AHL.
+
We first verify the circuits by artificially adding AHL.
−
 
+
−
figure (contrast of AHL and none)
+
  
 
We want to know the concentration of AHL and set a series of gradients. The results are as follows.
 
We want to know the concentration of AHL and set a series of gradients. The results are as follows.
  
−
figure (result)
+
https://static.igem.org/mediawiki/parts/8/82/T--NEU_CHINA--AHL_suoxiaoban.png
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 15:43, 20 October 2021


Plux_HS-LuxI-EGFP

In the AHL mediated quorum sensing system,after reaching the threshold, AHL can combine with endogenous LuxR to form a complex and activate the corresponding promoter PluxR. In this project, we selected the improved promoter Plux_HS of iGEM2018 Tsinghua.The promoter has the advantage of low leakage.After Plux_ HS is activated, the expressed AHL will combine with endogenous LuxR to form a complex and furtherly activate Plux_ HS promoter, so as to achieve the effect of cascade amplification, and finally express a large number of EGFP, making the detection effect significant.

We first verify the circuits by artificially adding AHL.

We want to know the concentration of AHL and set a series of gradients. The results are as follows.

T--NEU_CHINA--AHL_suoxiaoban.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 721
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1400