Difference between revisions of "Part:BBa K3735020"

Latest revision as of 15:42, 20 October 2021

Coding sequence

Bacterial 5’ untranslated regions as an important part of mRNA (5’ UTR), it involves in a complex regulation of gene expression; however, the exact sequence features contributing to gene regulation are not yet fully understood. But many researches have proved that 5’ UTR plays a central role in gene expression and protein production. At the DNA level, it involves in transcript formation for the interplay between promoter and initially transcribed sequences (ITS). But, at mRNA level, it influences transcript stability and translation because of secondary structure formation and so on. Therefor 5’ UTR is has a crucial contribution to the maintenance of a fine balance between transcription, transcript stability and translation. In the work of Simone Balzer Le, Ingerid Onsager et al, they design a novel 5’UTR-Dual UTR, utilizing the transcriptional and translational characteristics of 5’UTRs in a single expression cassette, which will improve protein expression greatly.

The Dual UTR contains two different UTRs which separated by a spacer region (Fig1). The 5’ UTR proximal to the promoter originates from the transcriptional screening(Tr-UTR) which affects transcript formation, while the second 5’ UTR is identified from the translational screening (Tn-UTR) which influences transcript stability and translation. And the spacer region provides enough space for physical separation of mutations affecting transcription and translation. By using the Dual UTR, we can improve the protein expression greatly.

Fig.1 Dual UTR(BBa_K3735020)

Sequence and Features