Difference between revisions of "Part:BBa K4013001"
Line 7: | Line 7: | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | It contains promoter region and RBS, which we treated as a complete element for inserting engineering gene expression in Synechocystis 6803. | |
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Line 17: | Line 17: | ||
<partinfo>BBa_K4013001 parameters</partinfo> | <partinfo>BBa_K4013001 parameters</partinfo> | ||
<!-- --> | <!-- --> | ||
+ | Characterization | ||
+ | These two native promoters are relatively weak in PCC 6803. We made this choice because excessive IAA would inhibit plant growth. For the terminator, we used a strong terminator called TrrnB. |
Revision as of 15:33, 20 October 2021
P0452
P0452 is an endogenous promoter of cyanobacteria PCC6803 with an RBS, so CDS can be added directly through the restriction endonuclease digestion site.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization
These two native promoters are relatively weak in PCC 6803. We made this choice because excessive IAA would inhibit plant growth. For the terminator, we used a strong terminator called TrrnB.