Difference between revisions of "Part:BBa K3829005"
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<br/><b>Fig.2</b> Representative images of the distribution of yeGPF. | <br/><b>Fig.2</b> Representative images of the distribution of yeGPF. | ||
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Revision as of 15:08, 20 October 2021
Signal peptide-SS
The signal peptide （SS） on the N terminus can lead the protein out of the cell.
Characterization
In our experiment, signal peptide (SS) was used. SS was a short peptide that could secrete proteins outside the cell. We constructed different gene circuits as shown in Figure 1 to verify the function of SS. The results showed that When SS was not present, fluorescence diffused in the cell (positive control). When SS was present (4609 and 5105), the fluorescence was displayed on the cell membrane, indicating that SS could secrete proteins outside the cell.
Fig.1 The construction of the gene circuit with Signal peptide (SS).
Fig.2 Representative images of the distribution of yeGPF.
Sequence and Features