Difference between revisions of "Part:BBa K3888005:Experience"

 
Line 1: Line 1:
  
 
__NOTOC__
 
__NOTOC__
−
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
−
how you used this part and how it worked out.
 
−
 
 
===Applications of BBa_K3888005===
 
===Applications of BBa_K3888005===
−
 
+
In order to achieve gene knockout, we used pK18mobsacB-Gm plasmid as a knockout vector. In addition, in order to achieve knockout, we selected about 1000bp sequences in the upstream and downstream of UppE and HupS respectively for design, amplified from the Rhodopseudomonas palustris CGA009 by PCR, and constructed knockout plasmids by the Gibson method (see the Design section for principles) ). The results of plasmid design and construction are as follows:
 +
[[File:UppE experiments.png]]<br>
 
===User Reviews===
 
===User Reviews===
 
<!-- DON'T DELETE --><partinfo>BBa_K3888005 StartReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K3888005 StartReviews</partinfo>

Revision as of 14:32, 20 October 2021


Applications of BBa_K3888005

In order to achieve gene knockout, we used pK18mobsacB-Gm plasmid as a knockout vector. In addition, in order to achieve knockout, we selected about 1000bp sequences in the upstream and downstream of UppE and HupS respectively for design, amplified from the Rhodopseudomonas palustris CGA009 by PCR, and constructed knockout plasmids by the Gibson method (see the Design section for principles) ). The results of plasmid design and construction are as follows: UppE experiments.png

User Reviews

UNIQca5964a445448b04-partinfo-00000000-QINU UNIQca5964a445448b04-partinfo-00000001-QINU