Difference between revisions of "Part:BBa K3829005"
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<p>In our experiment, signal peptide (SS) was used. SS was a short peptide that could secrete proteins outside the cell. We constructed different gene circuits as shown in Figure 1 to verify the function of SS. The results showed that When SS was not present, fluorescence diffused in the cell (positive control). When SS was present (4609 and 5105), the fluorescence was displayed on the cell membrane, indicating that SS could secrete proteins outside the cell.</p> | <p>In our experiment, signal peptide (SS) was used. SS was a short peptide that could secrete proteins outside the cell. We constructed different gene circuits as shown in Figure 1 to verify the function of SS. The results showed that When SS was not present, fluorescence diffused in the cell (positive control). When SS was present (4609 and 5105), the fluorescence was displayed on the cell membrane, indicating that SS could secrete proteins outside the cell.</p> | ||
| − | + | <img src="https://2021.igem.org/wiki/images/1/16/T--IvyMaker-China--Lab-03.jpg" style="width:75px;"> | |
| − | + | <img src="https://2021.igem.org/wiki/images/d/df/T--IvyMaker-China--Lab-04.jpg" style="width:20px;"> | |
<br/><b>Fig.1</b> The construction of the gene circuit with Signal peptide (SS). | <br/><b>Fig.1</b> The construction of the gene circuit with Signal peptide (SS). | ||
</figure> | </figure> | ||
Revision as of 14:31, 20 October 2021
Signal peptide-SS
The signal peptide （SS） on the N terminus can lead the protein out of the cell.
Characterization
In our experiment, signal peptide (SS) was used. SS was a short peptide that could secrete proteins outside the cell. We constructed different gene circuits as shown in Figure 1 to verify the function of SS. The results showed that When SS was not present, fluorescence diffused in the cell (positive control). When SS was present (4609 and 5105), the fluorescence was displayed on the cell membrane, indicating that SS could secrete proteins outside the cell.
<img src="
" style="width:75px;">
<img src="
" style="width:20px;">
Fig.1 The construction of the gene circuit with Signal peptide (SS).
</figure>
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]