Difference between revisions of "Part:BBa K3885112"

 
 
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UTR1 (BBa_K3885111) with mutation in RBS.
 
UTR1 (BBa_K3885111) with mutation in RBS.
  
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===Characterization===
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===Usage and Biology===
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The UTR1 mutation to UTR2 was used to construct P70a.b.c-UTR2.
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<p style="text-align: center;">Figure 1. Comparing expression in TXTL reactions to the model. Maximum rate of deGFP synthesis expressed from varying concentrations of P70a-UTR2-deGFP <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3885312">(BBa_K3885312)</a>,  P70b-UTR2-deGFP  <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3885322">(BBa_K3885322)</a>, P70c-UTR2-deGFP  <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3885332">(BBa_K3885332)</a> </p>
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Latest revision as of 13:25, 20 October 2021


UTR2

UTR1 (BBa_K3885111) with mutation in RBS.

Characterization

The UTR1 mutation to UTR2 was used to construct P70a.b.c-UTR2.

Figure 1. Comparing expression in TXTL reactions to the model. Maximum rate of deGFP synthesis expressed from varying concentrations of P70a-UTR2-deGFP (BBa_K3885312), P70b-UTR2-deGFP (BBa_K3885322), P70c-UTR2-deGFP (BBa_K3885332)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]