Difference between revisions of "Part:BBa K3752000"
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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[[File:T--Warwick--parentunannealed.png|600px|center]]
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NUPACK modelling was used to confirm the function of this part in silico as laboratory work could not be used to verify this due to time constraints.
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[[File:T--Warwick--parentunannealed.png|600px|center]]<br>
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As seen above, the natively folded state of the cgRNA does not present the dCas9 binding handle.<br>
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[[File:T--Warwick--parentannealed.png|600px|centre]]<br>
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Yet when the cgRNA anneals with the OXA-48 mRNA, the dCas9 binding handle (top in this image) is folded correctly, switching the CRISPRa system on.
 
<partinfo>BBa_K3752000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3752000 SequenceAndFeatures</partinfo>
  

Revision as of 12:49, 20 October 2021


OXA-48 Parent cgRNA

cgRNA which senses the mRNA of OXA-48 - used as a riboswitch by 2021 Warwick iGEM.

Sequence and Features

NUPACK modelling was used to confirm the function of this part in silico as laboratory work could not be used to verify this due to time constraints.

T--Warwick--parentunannealed.png

As seen above, the natively folded state of the cgRNA does not present the dCas9 binding handle.

T--Warwick--parentannealed.png

Yet when the cgRNA anneals with the OXA-48 mRNA, the dCas9 binding handle (top in this image) is folded correctly, switching the CRISPRa system on.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]