Difference between revisions of "Part:BBa K3885161"
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<partinfo>BBa_K3885161 short</partinfo> | <partinfo>BBa_K3885161 short</partinfo> | ||
− | This | + | This part contains a part of CXCL9 mRNA from human beings.<br> |
+ | This antimicrobial gene is part of a chemokine superfamily that encodes secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded is thought to be involved in T cell trafficking. The encoded protein binds to C-X-C motif chemokine 3 and is a chemoattractant for lymphocytes but not for neutrophils.<br> | ||
− | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | <html> | |
+ | In the project, we designed igRNA to hybridize with CXCL9 mRNA.<br> | ||
+ | To ensure the hybridization of CXCL9 mRNA and igRNA, we used<a href="http://www.nupack.org/">NUPACK webserver</a>.to analyze the 2D structure of target RNA.<br> | ||
+ | <style> | ||
+ | .flex{ | ||
+ | display: flex; | ||
+ | align-items: center; | ||
+ | justify-content: space-evenly; | ||
+ | } | ||
+ | </style> | ||
+ | <div class="flex" style="margin: 0 auto; width: 100%;"> | ||
+ | <div style="border: 1px solid #000;width: 45%; background-color: #f9f9f9;"> | ||
+ | <img src="https://static.igem.org/mediawiki/parts/8/86/2-111.png" width=98% height=70% style="display: block;margin: 10px auto;"/> | ||
+ | <p style="text-align"> </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | Three 39 bp single-strand sequences were selected as candidate hybridization region. Each hybridization region includes interacting sequence A and interacting sequence B. | ||
+ | <style> | ||
+ | .flex{ | ||
+ | display: flex; | ||
+ | align-items: center; | ||
+ | justify-content: space-evenly; | ||
+ | } | ||
+ | </style> | ||
+ | <div class="flex" style="margin: 0 auto; width: 100%;"> | ||
+ | <div style="border: 1px solid #000;width: 45%; background-color: #f9f9f9;"> | ||
+ | <img src="https://static.igem.org/mediawiki/parts/1/18/2-1122.png" width=98% height=70% style="display: block;margin: 10px auto;"/> | ||
+ | <p style="text-align"> Table 1. The segment used for testing. The three candidate hybridization regions are marked in green, blue and red. Interacting sequences are underlined.</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </html> | ||
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Revision as of 12:17, 20 October 2021
CXCL9 mRNA
This part contains a part of CXCL9 mRNA from human beings.
This antimicrobial gene is part of a chemokine superfamily that encodes secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded is thought to be involved in T cell trafficking. The encoded protein binds to C-X-C motif chemokine 3 and is a chemoattractant for lymphocytes but not for neutrophils.
Usage and Biology
In the project, we designed igRNA to hybridize with CXCL9 mRNA.
To ensure the hybridization of CXCL9 mRNA and igRNA, we usedNUPACK webserver.to analyze the 2D structure of target RNA.
Table 1. The segment used for testing. The three candidate hybridization regions are marked in green, blue and red. Interacting sequences are underlined.
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]