Difference between revisions of "Part:BBa K3886026"
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<partinfo>BBa_K3886026 short</partinfo> | <partinfo>BBa_K3886026 short</partinfo> | ||
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| + | <p>This composite part is used to achieve L-arabinose-induced Chromoprotein eforRed expression. We tested this circuit in our Hidro system. To learn more about Hidro system, please visit <a href="https://2021.igem.org/Team:NDNF_China/Proof_Of_Concept">NDNF_China Proof-Of-Concept</a>.</p> | ||
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| − | L-arabinose- | + | ===Characterization=== |
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| + | <p>To determine whether encapsulated bacteria in Hidro can respond to these external signal inputs, we encapsulated Hidro with the bacteria containing a genetic circuit that expresses chromoprotein (eforRed) in response to L-arabinose induction (Figure 1A). We then incubated the beads at 37 °C in the absence of L-arabinose or in the presence of 10mM L-arabinose. We found that encapsulated cells exposed to L-arabinose exhibited a pink colour compared with encapsulated cells not exposed to L-arabinose (Figure 1B). Thus, gene expression in Hidro can be exogenously controlled by chemical inducers. </p> | ||
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| + | <img src="https://2021.igem.org/File:T--NDNF_China--pocf26.png" alt="" width=600"> | ||
| + | <h6 style="text-align:center">Figure 1: (A) The schematic of a L-arabinose–controlled genetic switch to produce chromoprotein eforRed; (B) eforRed protein production in Hidro.</h6> | ||
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Revision as of 12:16, 20 October 2021
L-arabinose-inducible Chromoprotein eforRed expression circuits
This composite part is used to achieve L-arabinose-induced Chromoprotein eforRed expression. We tested this circuit in our Hidro system. To learn more about Hidro system, please visit NDNF_China Proof-Of-Concept.
Characterization
To determine whether encapsulated bacteria in Hidro can respond to these external signal inputs, we encapsulated Hidro with the bacteria containing a genetic circuit that expresses chromoprotein (eforRed) in response to L-arabinose induction (Figure 1A). We then incubated the beads at 37 °C in the absence of L-arabinose or in the presence of 10mM L-arabinose. We found that encapsulated cells exposed to L-arabinose exhibited a pink colour compared with encapsulated cells not exposed to L-arabinose (Figure 1B). Thus, gene expression in Hidro can be exogenously controlled by chemical inducers.
