Difference between revisions of "Part:BBa K3815008"
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<partinfo>BBa_K3815008 short</partinfo>
 
<partinfo>BBa_K3815008 short</partinfo>
  
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This part is made of Hist tag for the peptide production. After producing peptide with His tag, it is recovered by Ni chromatography. Finally, adding DTT to it, we cut the N terminal of Mxe GyrA intein. Then, we can get the targeted protein.In this part, the purpose protein is LL37 derived from human. This is antimicrobial peptide that can inhibit the growth of both gram-negative bacteria and gram-positive bacteria. In our experiment, we used it to kill the bacteria in the vase water.
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<h3><font size="4.5">Descriotion of this part</font> </h3>
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<h3><font size="3">Targeted protein</font> </h3>
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This part is for the purfication of antimicrobial peptide,LL37. This is derived from <i>Homo sapiens</i>. This can inhibit the growth of both the gram-positive and gram-negative bacteria. In our experiment, we used it to kill the bacteria in the vase water.<br><br>
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<h3><font size="3">Purification system</font> </h3>
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This part is composed of His tag, Mxe Gyr intein, PT linker, and targeted protein. This is for the peptide intein tag purification. Producing peptide with His tag, it is recovered by Ni chromatography. After that, adding DTT to it, the N terminal of Mxe GyrA intein is cut. Then, we get the targeted protein.　This method saves the time removing the tags compared to the method using only tags without intein.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:15, 20 October 2021


LL37-Mxe GryA intein-PT-linker-His tag

Descriotion of this part

Targeted protein

This part is for the purfication of antimicrobial peptide,LL37. This is derived from Homo sapiens. This can inhibit the growth of both the gram-positive and gram-negative bacteria. In our experiment, we used it to kill the bacteria in the vase water.

Purification system

This part is composed of His tag, Mxe Gyr intein, PT linker, and targeted protein. This is for the peptide intein tag purification. Producing peptide with His tag, it is recovered by Ni chromatography. After that, adding DTT to it, the N terminal of Mxe GyrA intein is cut. Then, we get the targeted protein.　This method saves the time removing the tags compared to the method using only tags without intein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 637
    Illegal AgeI site found at 130
    Illegal AgeI site found at 331
    Illegal AgeI site found at 765
  • 1000
    COMPATIBLE WITH RFC[1000]