Difference between revisions of "Part:BBa K3885171"

Revision as of 12:08, 20 October 2021 (view source) Kkarla (Talk | contribs) ← Older edit		Revision as of 12:09, 20 October 2021 (view source) Kkarla (Talk | contribs) (→‎Usage and Biology) Newer edit →
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	Based on the target gene(tetR), we chose CGG and AGG as PAM, and designed 5 gRNAs in the plasmid pTargetF (Figure 1). <br>		Based on the target gene(tetR), we chose CGG and AGG as PAM, and designed 5 gRNAs in the plasmid pTargetF (Figure 1). <br>
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	<span class='h3bb'>Sequence and Features</span>		<span class='h3bb'>Sequence and Features</span>

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Revision as of 12:09, 20 October 2021

Interacting Guide RNA（igRNA）

Guide RNA (gRNA) is a piece of RNAs that function as guides for RNA- or DNA-targeting enzymes, which they form complexes with. Very often these enzymes will delete, insert or otherwise alter the targeted DNA.

Usage and Biology

In the CRISPR/Cas9 system, gRNA can direct Cas9 protein to cut dsDNA at the sequence target site. Simultaneously, a hybridization region of igRNA is adding additional sequences in the 5' of sgRNA. Therefore, designing an active gRNA with high cleavage ability is important.
Based on the target gene(tetR), we chose CGG and AGG as PAM, and designed 5 gRNAs in the plasmid pTargetF (Figure 1).

Sequence and Features