Difference between revisions of "Part:BBa K3885171"
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<partinfo>BBa_K3885171 short</partinfo> | <partinfo>BBa_K3885171 short</partinfo> | ||
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+ | <div class="flex" style="margin: 0 auto; width: 100%;"> | ||
+ | <div style="border: 1px solid #000;width: 34%; background-color: #f9f9f9;"> | ||
+ | <img src="https://static.igem.org/mediawiki/parts/9/98/222_NUPANK-1.png" width=95% style="display: block;margin: 10px auto;"/> | ||
+ | <p style="text-align: center;"> </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | Guide RNA (gRNA) is a piece of RNAs that function as guides for RNA- or DNA-targeting enzymes, which they form complexes with. Very often these enzymes will delete, insert or otherwise alter the targeted DNA. <br> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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+ | <img src="https://static.igem.org/mediawiki/parts/c/c7/1-121.png" width=95% style="display: block;margin: 10px auto;"/> | ||
+ | <p style="text-align: center;"> Figure 1. Schematic of the design procedure of the gRNA construction and screening. There are five 20nt sequences to change into five different gRNAs.</p> | ||
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+ | In the CRISPR/Cas9 system, gRNA can direct Cas9 protein to cut dsDNA at the sequence target site. Simultaneously, a hybridization region of igRNA is adding additional sequences in the 5' of sgRNA. Therefore, designing an active gRNA with high cleavage ability is important.<br> | ||
+ | Based on the target gene(tetR), we chose CGG and AGG as PAM, and designed 5 gRNAs in the plasmid pTargetF (Figure 1). <br> | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Revision as of 12:07, 20 October 2021
Interacting Guide RNA(igRNA)
Usage and Biology
Figure 1. Schematic of the design procedure of the gRNA construction and screening. There are five 20nt sequences to change into five different gRNAs.
Based on the target gene(tetR), we chose CGG and AGG as PAM, and designed 5 gRNAs in the plasmid pTargetF (Figure 1).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]