Difference between revisions of "Part:BBa K3888004:Design"
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<partinfo>BBa_K3888004 short</partinfo>
 
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[[File:pET28-pTac-lacO-hydrogenase .png]]
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===Figure 1: The Oxygen-tolerant Hydrogenase introduction plasmid constructed, which has a length of 8261bp.===
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HoxK1 encodes the small subunit of oxygen-tolerant hydrogenase, and it's controlled by a Lac operator.
 
<partinfo>BBa_K3888004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3888004 SequenceAndFeatures</partinfo>
  

Revision as of 12:04, 20 October 2021


HoxK1

PET28-pTac-lacO-hydrogenase .png

Figure 1: The Oxygen-tolerant Hydrogenase introduction plasmid constructed, which has a length of 8261bp.

HoxK1 encodes the small subunit of oxygen-tolerant hydrogenase, and it's controlled by a Lac operator.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 940
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Change signal sequence


Source

Hydrogenovibrio marinus MH-110 Genome

References

1. Kim, Jaoon YH, Byung Hoon Jo, and Hyung Joon Cha. "Production of biohydrogen by heterologous expression of oxygen-tolerant Hydrogenovibrio marinus [NiFe]-hydrogenase in Escherichia coli." Journal of biotechnology 155.3 (2011): 312-319.