Difference between revisions of "Part:BBa K3815006"
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<partinfo>BBa_K3815006 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3815006 SequenceAndFeatures</partinfo> | ||
==Purification== | ==Purification== | ||
| − | [[File:Engineering 203 SDS- | + | [[File:Engineering 203 SDS-PAGE②.png|300px|thumb|right|Fig1. SDS-PAGE of purified peptide ]] |
<h3><font size="4.5">Expression</font> </h3> | <h3><font size="4.5">Expression</font> </h3> | ||
<ul> | <ul> | ||
Revision as of 12:00, 20 October 2021
CecropineA-Mxe GryA intein-PT-linker-His tag
Descriotion of this part
Targeted protein
This part is for the purfication of antimicrobial peptide, CecropinA. This is derived from Hyalophora cecropia. This can inhibit the growth of both gram-negative bacteria and gram-positive bacteria. In our experiment, we used it to kill the bacteria in the vase water.
Purification system
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 67
Illegal AgeI site found at 346 - 1000COMPATIBLE WITH RFC[1000]
Purification
Expression
- Cells were grown in 1000ml LB media at 37oC shaking at 180 rpm.
- when the OD exceeded 0.35, 1 M IPTG 2ml was added to induce the peptide expression.
- Incubate at 30℃ at 180rpm for 6 hours after adding IPTG.
Purification
1.When this fused protein were produced, it self-assembled and precipitated
2.The aggregate was collected by centrifugation.
3.Adding 40mM DTT to this aggregate, the targeted protein was cut out by the cleavage of intein.
4.SDSPAGE was performed in order to confirm the presence of it.
Fig1 shows the result of SDS-PAGE.
The lane 1, 5,and 9 are the result of CecropinA.
CecropinA is 4051Da, so these date shows that we could not confirm CecA production.