Difference between revisions of "Part:BBa K3846359"
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<partinfo>BBa_K3846359 short</partinfo> | <partinfo>BBa_K3846359 short</partinfo> | ||
| − | + | Humulene is the precursor of the sesquiterpene ketone zerumbone, which has immunoregulatory and anticancer activities. The biosynthesis of cyclic sesquiterpenes is catalysed by FPP. In the case of humulene biosynthesis, this cyclase is alpha humulene synthase. | |
| + | This part describes the IPTG-inducible expression cassette of alpha humulene synthase using an T7 promoter. | ||
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<partinfo>BBa_K3846359 parameters</partinfo> | <partinfo>BBa_K3846359 parameters</partinfo> | ||
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| + | ===iGEM Hamburg 2021 part collection=== | ||
| + | Terpenoids are an important group of natural products used as biofuels, drugs or fragrances. Naturally occuring in plants it has been shown that microbial terpene production in microorganisms like yeast, E. coli or cyanobacteria is possible. | ||
| + | Nevertheless iGEM projects seem to rarely focus on this interesting class of natural products which is correlated with a lack of useful parts inside the iGEM registry. | ||
| + | |||
| + | Fortunately we were able to change that and designed a novel golden gate based toolbox which allows. | ||
| + | <ol style="list-style-type:lower-alpha"> | ||
| + | <li>production of terpenoid precursors and simple terpenoids</li> | ||
| + | <li>creation of CYP P450-reductase fusion enzymes to optimise processing of terpenoid precursors and production of bioactive target products</li> | ||
| + | <li>modularity of the system to enable exchange of linker sequences/promoters/etc. (MoClo-compatible toolbox)</li> | ||
| + | </ol> | ||
| + | |||
| + | ===MoClo-based Part Design 2.0=== | ||
| + | <p>To improve the usefulness of our parts, we then aimed to make our parts compatible with the MoClo standard of goten gate based IIS restriction enzyme assembly. Thereby we expanded the Common Genetic Syntax for fusion sites to allow the creation of a) fusion proteins connected by linker sequences and b) multiple CDS expressed in an operon. | ||
| + | More useful information and an overview of all our parts can be found on our [https://2021.igem.org/Team:Hamburg/Part_Collection wiki]. | ||
| + | |||
| + | [[File:T--Hamburg--parts overview MolClo.png|600px|thumb|left|'''Figure 1''': <b> MoClo syntax of the part collection. </b> <br>]] | ||
Latest revision as of 11:49, 20 October 2021
Humulen synthase expression casette (T7 promoter)
Humulene is the precursor of the sesquiterpene ketone zerumbone, which has immunoregulatory and anticancer activities. The biosynthesis of cyclic sesquiterpenes is catalysed by FPP. In the case of humulene biosynthesis, this cyclase is alpha humulene synthase. This part describes the IPTG-inducible expression cassette of alpha humulene synthase using an T7 promoter.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
iGEM Hamburg 2021 part collection
Terpenoids are an important group of natural products used as biofuels, drugs or fragrances. Naturally occuring in plants it has been shown that microbial terpene production in microorganisms like yeast, E. coli or cyanobacteria is possible. Nevertheless iGEM projects seem to rarely focus on this interesting class of natural products which is correlated with a lack of useful parts inside the iGEM registry.
Fortunately we were able to change that and designed a novel golden gate based toolbox which allows.
- production of terpenoid precursors and simple terpenoids
- creation of CYP P450-reductase fusion enzymes to optimise processing of terpenoid precursors and production of bioactive target products
- modularity of the system to enable exchange of linker sequences/promoters/etc. (MoClo-compatible toolbox)
MoClo-based Part Design 2.0
To improve the usefulness of our parts, we then aimed to make our parts compatible with the MoClo standard of goten gate based IIS restriction enzyme assembly. Thereby we expanded the Common Genetic Syntax for fusion sites to allow the creation of a) fusion proteins connected by linker sequences and b) multiple CDS expressed in an operon. More useful information and an overview of all our parts can be found on our wiki.
