Difference between revisions of "Part:BBa K3734015"

 
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<partinfo>BBa_K3734015 short</partinfo>
 
<partinfo>BBa_K3734015 short</partinfo>
  
 
Insulin is a kind of protein that can make blood glucose level down.
 
Insulin is a kind of protein that can make blood glucose level down.
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                Insulin
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            <div class="pageContent-main__textBox">
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                <p>We have proved that Insulin can be processed and secreted outside the cell in 293T cells.
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            <h2 class="pageContent-main__title pageContent-main__subtitle">
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                1.Pattern diagram
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                <p style="text-align: center;">
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                    <img width="400px" src="https://2021.igem.org/wiki/images/f/f2/T--CSU_CHINA--tupian29.png"></p>
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                <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.1 The model diagram of 9XUAS-Insulin</p>
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            <h2 class="pageContent-main__title pageContent-main__subtitle">
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                2.Experiment
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                2.1 Method
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                <p>In the experiment, we used an ELIAS kit specially designed to detect human mature insulin to detect cell eliquidation.
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                <!--put text here, <p>content</p>-->
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                2.2 Results
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            <div class="pageContent-main__textBox">
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                <p>Insulin can be processed and secreted outside the cell in 293T cells.
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                </p>
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                <img class="image" src="https://2021.igem.org/wiki/images/5/58/T--CSU_CHINA--tupian30.png">
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                <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.2 Standard curve of absorbance and insulin concentration</p>
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                <img class="image" src="https://2021.igem.org/wiki/images/5/57/T--CSU_CHINA--tupian31.png">
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                <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.3 Insulin concentration in supernatant after blue light irradiation and dark treatment respectively</p>
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                <img class="image" src="https://2021.igem.org/wiki/images/6/6c/T--CSU_CHINA--tupian32.png">
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                <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.4 Insulin concentration in supernatant (excluding insulin in medium) after blue light irradiation and dark treatment respectively</p>
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            <h2 class="pageContent-main__title pageContent-main__subtitle">
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                3.Caution
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                <p>Insulin is not suitable for long time preservation under the best culture temperature of cells (37℃). In the mean time, cells would also consume some of the expressed insulin. So the best tst time for Insulin is approximately 24 hours
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                    after transfection, it is relatively shorter than the test interval of LUC.
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            <h2 class="pageContent-main__title pageContent-main__subtitle">
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                Reference：
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                <p>[1]Mingqi Xie, Haifeng Ye, Hui Wang.β-cell-mimetic designer cells provide closed-loop glycemic control[J].Science.2016 Dec 9;354(6317):1296-1301.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 11:29, 20 October 2021

Insulin

Insulin is a kind of protein that can make blood glucose level down.

Insulin

We have proved that Insulin can be processed and secreted outside the cell in 293T cells.

1.Pattern diagram

Fig.1 The model diagram of 9XUAS-Insulin

2.Experiment

2.1 Method

In the experiment, we used an ELIAS kit specially designed to detect human mature insulin to detect cell eliquidation.

2.2 Results

Insulin can be processed and secreted outside the cell in 293T cells.

Fig.2 Standard curve of absorbance and insulin concentration

Fig.3 Insulin concentration in supernatant after blue light irradiation and dark treatment respectively

Fig.4 Insulin concentration in supernatant (excluding insulin in medium) after blue light irradiation and dark treatment respectively

3.Caution

Insulin is not suitable for long time preservation under the best culture temperature of cells (37℃). In the mean time, cells would also consume some of the expressed insulin. So the best tst time for Insulin is approximately 24 hours after transfection, it is relatively shorter than the test interval of LUC.

Reference：

[1]Mingqi Xie, Haifeng Ye, Hui Wang.β-cell-mimetic designer cells provide closed-loop glycemic control[J].Science.2016 Dec 9;354(6317):1296-1301.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]