Difference between revisions of "Part:BBa K3734014"

LUC is a reported gene in the luminescence system of fluorease. It comes from fireflies and characterizes the amount of gene expression in the target by detecting
 the luminescence value of its excited fluorescent hormone at a wavelength of 560nm. Let's take LUC after CHREBP promoter as an example to prove that LUC can
 complete the task of reporting genes well.

Fig.1 The model diagram of CHREBP-LUC

We used LUC as report genes to reflect the level of expression through detecting luminescence value at 560nm wavelength and the error REN luminescence caused by the number of eliminated cells.

The experiments proved that LUC can complete the mission of being a report gene well.

Fig.2 The expression level of inducible promoter CHREBP was respectively analyzed at 48h in 25mM, 5.6mm and 0mM glucose culture

Try to convert REN at the same time to adjust the errors caused by other factors such as different cell numbers and cell states caused by different culture conditions.