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                 <p>LUC is a reported gene in the luminescence system of fluorease. It comes from fireflies and characterizes the amount of gene expression in the target by detecting the luminescence value of its excited fluorescent hormone at a wavelength of 560nm. Let's take LUC after CHREBP promoter as an example to prove that LUC can complete the task of reporting genes well.
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                 <p>LUC is a reported gene in the luminescence system of fluorease. It comes from fireflies and characterizes the amount of gene expression in the target by <br> detecting the luminescence value of its excited fluorescent hormone at a wavelength of 560nm. Let's take LUC after CHREBP promoter as an example to prove that LUC can complete the task of reporting genes well.
 
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Revision as of 11:24, 20 October 2021


LUC

Luciferin(LUC) is a protein that can activate fluorescein.We use it as reporter gene.

LUC

LUC is a reported gene in the luminescence system of fluorease. It comes from fireflies and characterizes the amount of gene expression in the target by
 detecting the luminescence value of its excited fluorescent hormone at a wavelength of 560nm. Let's take LUC after CHREBP promoter as an example to prove that LUC can complete the task of reporting genes well.

1.Pattern diagram

Fig.1 The model diagram of CHREBP-LUC

2.Experiments

2.1 Method

We used LUC as report genes to reflect the level of expression through detecting luminescence value at 560nm wavelength and the error REN luminescence caused by the number of eliminated cells.

2.2 Results

The experiments proved that LUC can complete the mission of being a report gene well.

Fig.2 The expression level of inducible promoter CHREBP was respectively analyzed at 48h in 25mM, 5.6mm and 0mM glucose culture

3.Caution

Try to convert REN at the same time to adjust the errors caused by other factors such as different cell numbers and cell states caused by different culture conditions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 64
    Illegal NgoMIV site found at 1408
    Illegal NgoMIV site found at 1429
    Illegal AgeI site found at 1132
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1314