Difference between revisions of "Part:BBa K3805999:Design"
Line 1: Line 1:
−
这里主要是写怎么设计的以及设计的思路
+
__NOTOC__
 +
<partinfo>BBa_K3805999 short</partinfo>
 +
 
 +
<partinfo>BBa_K3805999 SequenceAndFeatures</partinfo>
 +
 
 +
==Designed Notes==
 +
 
 +
To ensure the specificity of binding, 19bp sequence on the T7 promoter was selected as sgRNA. We design the special sgRNA on website http://crispr.dfci.harvard.edu/SSC/ which is owned by Harvard.
 +
 
 +
==Source==
 +
 
 +
We expect to synthesize this fragment.

Revision as of 11:11, 20 October 2021

sgRNA bind to T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Designed Notes

To ensure the specificity of binding, 19bp sequence on the T7 promoter was selected as sgRNA. We design the special sgRNA on website http://crispr.dfci.harvard.edu/SSC/ which is owned by Harvard.

Source

We expect to synthesize this fragment.