Difference between revisions of "Part:BBa K091146:Experience"

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This part was tested with the autoinducer molecules 3OC6 and 3OC12 in cells that constitutively expressed LuxR and LasR. It was expected that GFP would be expressed when 3OC12 was given to the cells but not when both 3OC12 and 3OC6 was given. This was because of the lux box that was put in the -35 to -10 region of the promoter. Binding of LuxR+3OC6 to this region was expected to result in repression of the promoter. I found that 3OC12 appeared to activate LuxR, which resulted in unexpected behavior. See the graphs below for my data.  

This part was tested with the autoinducer molecules 3OC6 and 3OC12 in cells that constitutively expressed LuxR and LasR. It was expected that GFP would be expressed when 3OC12 was given to the cells but not when both 3OC12 and 3OC6 was given. This was because of the lux box that was put in the -35 to -10 region of the promoter. Binding of LuxR+3OC6 to this region was expected to result in repression of the promoter. I found that 3OC12 appeared to activate LuxR, which resulted in unexpected behavior. See the graphs below for my data.  

[[Image:Graph1 WD.png|200px|thumb|none|Test]]

[[Image:Graph1 WD.png|200px|thumb|none|Test]]

[[Image:Graph2 WD.png|200px|thumb|none|Test]]

[[Image:Graph2 WD.png|200px|thumb|none|Test]]

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