Difference between revisions of "Part:BBa K3989014"
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<partinfo>BBa_K3989014 short</partinfo> | <partinfo>BBa_K3989014 short</partinfo> | ||
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In this construct, outer membrane-associated protein ClyA ([[Part:BBa_K811000]]) is used to display the double cellulose binding domain (dCBD, [[Part:BBa_K1321340]]) to the surface of bacterial outer membrane vesicles (OMVs). To make dCBD better displayed, we put multiple linkers and a 3×FLAG tag between ClyA and dCBD to minimize the steric hindrance. | In this construct, outer membrane-associated protein ClyA ([[Part:BBa_K811000]]) is used to display the double cellulose binding domain (dCBD, [[Part:BBa_K1321340]]) to the surface of bacterial outer membrane vesicles (OMVs). To make dCBD better displayed, we put multiple linkers and a 3×FLAG tag between ClyA and dCBD to minimize the steric hindrance. | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | ==Chracterization by team 2021 UZurich== | ||
[[File:ClyA-dCBD-SGI.png|500px]] | [[File:ClyA-dCBD-SGI.png|500px]] | ||
Revision as of 10:30, 20 October 2021
double cellulose binding domain fused with ClyA for OMV display
In this construct, outer membrane-associated protein ClyA (Part:BBa_K811000) is used to display the double cellulose binding domain (dCBD, Part:BBa_K1321340) to the surface of bacterial outer membrane vesicles (OMVs). To make dCBD better displayed, we put multiple linkers and a 3×FLAG tag between ClyA and dCBD to minimize the steric hindrance.
Usage and Biology
Chracterization by team 2021 UZurich
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]