Difference between revisions of "Part:BBa K3989014"

Revision as of 10:28, 20 October 2021

double cellulose binding domain fused with ClyA for OMV display In this construct, outer membrane-associated protein ClyA (Part:BBa_K811000) is used to display the double cellulose binding domain (dCBD, Part:BBa_K1321340) to the surface of bacterial outer membrane vesicles (OMVs). To make dCBD better displayed, we put multiple linkers and a 3×FLAG tag between ClyA and dCBD to minimize the steric hindrance.

Usage and Biology

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K3989014 short</partinfo>
-In this construct, outer membrane-associated protein ClyA ([[Part:BBa_K811000]]) is used to display the double cellulose binding domain (dCBD, [[Part:BBa_K3989023]]) to the surface of bacterial outermembrane vesicles (OMVs) to trigger plant immune responses. To make elf18 better recognized by plant immune receptor FLS2, we put mutiple linkers and a 3×FLAG tag between ClyA and elf18 to minimize the steric hindrance.
+In this construct, outer membrane-associated protein ClyA ([[Part:BBa_K811000]]) is used to display the double cellulose binding domain (dCBD, [[Part:BBa_K1321340]]) to the surface of bacterial outer membrane vesicles (OMVs). To make dCBD better displayed, we put multiple linkers and a 3×FLAG tag between ClyA and dCBD to minimize the steric hindrance.
 ===Usage and Biology===