Difference between revisions of "Part:BBa K3888003:Design"
Line 3: Line 3:
 
<partinfo>BBa_K3888003 short</partinfo>
 
<partinfo>BBa_K3888003 short</partinfo>
  
−
[[File:T--SJTang--BBa_K3888011-1.png]]
+
[[File:pET28-pTac-lacO-hydrogenase .png]]
−
==Figure 1: The Oxygen-tolerant Hydrogenase introduction plasmid constructed, which has a length of 8261bp.==
+
===Figure 1: The Oxygen-tolerant Hydrogenase introduction plasmid constructed, which has a length of 8261bp.===
  
 
HoxG1 encodes the large subunit of oxygen-tolerant hydrogenase, and it's controlled by a Lac operator. Overall, it's clear to see that this gene is located between 5094bp and 6926bp.
 
HoxG1 encodes the large subunit of oxygen-tolerant hydrogenase, and it's controlled by a Lac operator. Overall, it's clear to see that this gene is located between 5094bp and 6926bp.

Revision as of 10:26, 20 October 2021


HoxG1

PET28-pTac-lacO-hydrogenase .png

Figure 1: The Oxygen-tolerant Hydrogenase introduction plasmid constructed, which has a length of 8261bp.

HoxG1 encodes the large subunit of oxygen-tolerant hydrogenase, and it's controlled by a Lac operator. Overall, it's clear to see that this gene is located between 5094bp and 6926bp.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 282
    Illegal XbaI site found at 384
    Illegal SpeI site found at 450
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 450
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 597
    Illegal BamHI site found at 1512
    Illegal XhoI site found at 1834
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 282
    Illegal XbaI site found at 384
    Illegal SpeI site found at 450
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 282
    Illegal XbaI site found at 384
    Illegal SpeI site found at 450
    Illegal AgeI site found at 1728
  • 1000
    COMPATIBLE WITH RFC[1000]

Design Notes

Codon optimization for E. coli


Source

Hydrogenovibrio marinus MH-110 Genome

References

1. Kim, Jaoon YH, Byung Hoon Jo, and Hyung Joon Cha. "Production of biohydrogen by heterologous expression of oxygen-tolerant Hydrogenovibrio marinus [NiFe]-hydrogenase in Escherichia coli." Journal of biotechnology 155.3 (2011): 312-319.