Difference between revisions of "Part:BBa K3989014"
| Line 2: | Line 2: | ||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K3989014 short</partinfo> | <partinfo>BBa_K3989014 short</partinfo> | ||
| − | + | In this construct, outer membrane-associated protein ClyA ([[Part:BBa_K811000]]) is used to display the double cellulose binding domain (dCBD, [[Part:BBa_K3989023]]) to the surface of bacterial outermembrane vesicles (OMVs) to trigger plant immune responses. To make elf18 better recognized by plant immune receptor FLS2, we put mutiple linkers and a 3×FLAG tag between ClyA and elf18 to minimize the steric hindrance. | |
| − | + | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 10:25, 20 October 2021
double cellulose binding domain fused with ClyA for OMV display
In this construct, outer membrane-associated protein ClyA (Part:BBa_K811000) is used to display the double cellulose binding domain (dCBD, Part:BBa_K3989023) to the surface of bacterial outermembrane vesicles (OMVs) to trigger plant immune responses. To make elf18 better recognized by plant immune receptor FLS2, we put mutiple linkers and a 3×FLAG tag between ClyA and elf18 to minimize the steric hindrance.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]