Difference between revisions of "Part:BBa K3765000:Design"
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===References=== | ===References=== | ||
# Zakeri, B. et al. Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. ''Proc. Natl Acad. Sci. USA'' '''109''', E690–E697 (2012). | # Zakeri, B. et al. Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. ''Proc. Natl Acad. Sci. USA'' '''109''', E690–E697 (2012). | ||
− | + | # Sutherland, A. R., Alam, M. K. & Geyer, C. R. Post-translation assembly of protein parts into complex devices using SpyTag/SpyCatcher protein ligase. ''Chembiochem'' '''20''', 319–328 (2019). | |
− | # Sutherland, A. R., Alam, M. K. & Geyer, C. R. Post-translation assembly of protein parts into complex devices using SpyTag/SpyCatcher protein ligase. ''Chembiochem'' '''20''', 319–328 (2019). | + | # Keeble, A. H. et al. Evolving accelerated amidation by SpyTag/SpyCatcher to analyze membrane dynamics. ''Angew. Chem. Int. Ed.'' '''56''', 16521–16525 (2017). |
− | + | ||
− | Keeble, A. H. et al. Evolving accelerated amidation by SpyTag/SpyCatcher to analyze membrane dynamics. ''Angew. Chem. Int. Ed.'' '''56''', 16521–16525 (2017). | + |
Latest revision as of 10:14, 20 October 2021
SpyTag002
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimized for expression in E. coli.
Source
Modified peptide. Engineered from the second immunoglobulin-like collagen adhesin domain (CnaB2) of the fibronectin binding protein FbaB from Streptococcus pyogenes.
References
- Zakeri, B. et al. Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. Proc. Natl Acad. Sci. USA 109, E690–E697 (2012).
- Sutherland, A. R., Alam, M. K. & Geyer, C. R. Post-translation assembly of protein parts into complex devices using SpyTag/SpyCatcher protein ligase. Chembiochem 20, 319–328 (2019).
- Keeble, A. H. et al. Evolving accelerated amidation by SpyTag/SpyCatcher to analyze membrane dynamics. Angew. Chem. Int. Ed. 56, 16521–16525 (2017).