Difference between revisions of "Part:BBa K4032105"

Line 5: Line 5:
 
Contents:
 
Contents:
  
・The lac promoter (https://parts.igem.org/Part:BBa_R0010)
+
・The lac promoter (from <partinfo>BBa_R0010</partinfo>)
  
・The  RBS (https://parts.igem.org/Part:BBa_B0034)
+
・The  RBS (from <partinfo>BBa_B0034<//partinfo>)
  
 
・The gene of DegP protein.
 
・The gene of DegP protein.
  
・The double terminator (https://parts.igem.org/Part:BBa_B0015)
+
・The double terminator (from <partinfo>BBa_B0015</partinfo>)
  
  
Line 25: Line 25:
 
===Designs===
 
===Designs===
  
The lac promoter , the RBS and the double terminator are added to BBa_K40320xx, forming this part.
+
The lac promoter , the RBS and the double terminator are added to <partinfo>BBa_K40320xx</partinfo>, forming this part.
  
The lac promoter , the RBS and the double terminator are used from BBa_J04450 (https://parts.igem.org/Part:BBa_J04450) .
+
The lac promoter , the RBS and the double terminator are used from <partinfo>BBa_J04450</partinfo>.
  
 
This part was created by the In-Fusion method.
 
This part was created by the In-Fusion method.

Revision as of 09:56, 20 October 2021


lacI+RBS+DegP+double terminator

Contents:

・The lac promoter (from BBa_R0010)

・The RBS (from No part name specified with partinfo tag.)


Usage and Biology

DegP is a type of heat shock protein in E.coli. Heat shock protein is a family of proteins whose expression is induced by SOS responses such as heat.

It is also a type of serine protease due to the presence of serine residues in the active center.

In particular, it recognizes and degrades peptides whose structure has been damaged by heat or misfolding.


Designs

The lac promoter , the RBS and the double terminator are added to No part name specified with partinfo tag., forming this part.

The lac promoter , the RBS and the double terminator are used from BBa_J04450.

This part was created by the In-Fusion method.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1364
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1364
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1364
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1364
    Illegal AgeI site found at 2206
    Illegal AgeI site found at 2318
  • 1000
    COMPATIBLE WITH RFC[1000]