Difference between revisions of "Part:BBa K3766034"

Revision as of 09:35, 20 October 2021

Synthetic RBS designed for sfGFP

This part is a synthetic RBS designed for sfGFP(BBa_K2675005) using the Salis Lab / De Novo DNA RBS Library Calculator v2.1.1 [1, 2, 3].

Usage and Biology

This RBS was used to drive the expression of sfGFP(BBa_K2675005) under the control of the T7 promoter (BBa_K2150031 promoter in the composite part BBa_K3766111.

It was obtained upon cloning from a library of 16 potential RBS sequences (GSCKTACATTAMAATAAGKAGGTCCAA) having a translation initiation rate in the RBS library of minimum 166.18 and maximum 35865.19 (using the RBS Calculator's proportional scale).

References

[1] Reis AC, Salis HM. An automated model test system for systematic development and improvement of gene expression models. ACS synthetic biology (2020) 9: 3145–3156.

[2] Farasat I, Kushwaha M, Collens J, Easterbrook M, Guido M, Salis HM. Efficient search, mapping, and optimization of multi-protein genetic systems in diverse bacteria. Molecular Systems Biology (2014) 10: 731.

[3] Ng CY, Farasat I, Maranas CD, Salis HM. Rational design of a synthetic Entner-Doudoroff pathway for improved and controllable NADPH regeneration. Metabolic Engineering (2015) 29: 86–96.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3766034 short</partinfo>
-sfGFP RBS
+This part is a synthetic RBS designed for sfGFP([[Part:BBa_K2675005|BBa_K2675005]]) using the [https://www.denovodna.com/ Salis Lab / De Novo DNA RBS Library Calculator v2.1.1] [1, 2, 3].
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+This RBS was used to drive the expression of sfGFP([[Part:BBa_K2675005|BBa_K2675005]]) under the control of the T7 promoter ([[Part:BBa_K2150031|BBa_K2150031]] promoter in the composite part [[Part:BBa_K3766111|BBa_K3766111]].
+It was obtained upon cloning from a library of 16 potential RBS sequences (GSCKTACATTAMAATAAGKAGGTCCAA) having a translation initiation rate in the RBS library of minimum 166.18 and maximum 35865.19 (using the RBS Calculator's proportional scale).
+===References===
+[1] Reis AC, Salis HM. An automated model test system for systematic development and improvement of gene expression models. ACS synthetic biology (2020) 9: 3145–3156.
+[2] Farasat I, Kushwaha M, Collens J, Easterbrook M, Guido M, Salis HM. Efficient search, mapping, and optimization of multi-protein genetic systems in diverse bacteria. Molecular Systems Biology (2014) 10: 731.
+[3] Ng CY, Farasat I, Maranas CD, Salis HM. Rational design of a synthetic Entner-Doudoroff pathway for improved and controllable NADPH regeneration. Metabolic Engineering (2015) 29: 86–96.
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