Difference between revisions of "Part:BBa K4032006"
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<partinfo>BBa_K4032006 short</partinfo>
 
<partinfo>BBa_K4032006 short</partinfo>
  
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NC_000913.3 malS alpha-amylase [Escherichia coli str. K-12 substr. MG1655] - Gene - NCBI (nih.gov)
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NC_000913.3 https://www.ncbi.nlm.nih.gov/gene/948088
  
 
Contents :
 
Contents :
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Usage and Biology
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===Usage and Biology===
  
 
This enzyme hydrolyzes of (1-4)-alpha-D-glycosidic linkages in polysaccharides containing three or more (1-4)-alpha-linked D-glucose units. See details NC_000913.3 (https://www.ncbi.nlm.nih.gov/gene/948088).
 
This enzyme hydrolyzes of (1-4)-alpha-D-glycosidic linkages in polysaccharides containing three or more (1-4)-alpha-linked D-glucose units. See details NC_000913.3 (https://www.ncbi.nlm.nih.gov/gene/948088).

Revision as of 09:28, 20 October 2021


RBS+amylase-RFP

NC_000913.3 https://www.ncbi.nlm.nih.gov/gene/948088

Contents :

・The gene codes for the amylase-RFP fusion protein.

・The native RBS(Part:BBa K523001 - parts.igem.org)


Usage and Biology

This enzyme hydrolyzes of (1-4)-alpha-D-glycosidic linkages in polysaccharides containing three or more (1-4)-alpha-linked D-glucose units. See details NC_000913.3 (https://www.ncbi.nlm.nih.gov/gene/948088).

In the case of this part, Red red fluorescence of RFP is observed under the fluorescence microscope, but isn’t observed under natural light.


The lac promoter and double terminator are added to this part forming BBa_K4032xx .


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1233
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 369
    Illegal AgeI site found at 1534
    Illegal AgeI site found at 2613
    Illegal AgeI site found at 2725
  • 1000
    COMPATIBLE WITH RFC[1000]