Difference between revisions of "Part:BBa K3887012"

Latest revision as of 08:45, 20 October 2021

sgRNA2

Single guide RNA sequence we designed for TnaA knockout in E.coli BL21(DE3).

Single guide RNA (sgRNA) is used during gene editing process. It is the sequences of RNA molecules that bind to the target location. It is made of crRNA and trcrRNA. During the editing process, tracrRNA first recognizes the Cas9 and binds to it. The Cas9 receives sequence information from the crRNA and moves to the target location to perform the cleaving of DNA.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3887012 short</partinfo>
-Guide RNA we designed for TnaA gene knockout in <i>E.coli</i> BL21(DE3).
+Single guide RNA sequence we designed for TnaA knockout in E.coli BL21(DE3).
+Single guide RNA (sgRNA) is used during gene editing process. It is the sequences of RNA molecules that bind to the target location. It is made of crRNA and trcrRNA. During the editing process, tracrRNA first recognizes the Cas9 and binds to it. The Cas9 receives sequence information from the crRNA and moves to the target location to perform the cleaving of DNA.
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