Difference between revisions of "Part:BBa K3734004"

Revision as of 08:14, 20 October 2021

GI

GI is a kind of light-activated protein. If you shine blue light on it, it will change its own structure and will be able to combined with LOV.

VP16

VP16 is a transcription activation factor, also commonly used in double yeast hybrid system. It can realize the activation of downstream UAS transcription. It is usually used together with GAL4 combining domain, which can recognize and combine with UAS, to locate UAS.

1.Pattern diagram

Fig.1 The model diagram of LOV-VP16

2.Caution

GAL4 combining domain is universal among eukaryotic cells, but the activation factor needs to be chosen differently according to the chassis creature.

Reference：

[1]Chun Jeih Ryu , Charles E Whitehurst, Jianzhu Chen.Expression of Gal4-VP16 and Gal4-DNA binding domain under the control of the T lymphocyte-specific lck proximal promoter in transgenic mice[J].BMB Rep. 2008 Aug 31;41(8):575-80.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 3147
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 3042
Illegal XhoI site found at 695
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1197
Illegal AgeI site found at 2424
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1821

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                  <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.1 The model diagram of LOV-VP16</p>