Difference between revisions of "Part:BBa K3734008"
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VP16 is a kind of protein that can activate downstream gene expression. | VP16 is a kind of protein that can activate downstream gene expression. | ||
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Revision as of 08:10, 20 October 2021
VP16
VP16 is a kind of protein that can activate downstream gene expression.
VP16
VP16 is a transcription activation factor, also commonly used in double yeast hybrid system. It can realize the activation of downstream UAS transcription. It is usually used together with GAL4 combining domain, which can recognize and combine with UAS, to locate UAS.
1.Pattern diagram
Fig.1 The model diagram of LOV-VP16
2.Caution
GAL4 combining domain is universal among eukaryotic cells, but the activation factor needs to be chosen differently according to the chassis creature.
Reference：
[1]Chun Jeih Ryu , Charles E Whitehurst, Jianzhu Chen.Expression of Gal4-VP16 and Gal4-DNA binding domain under the control of the T lymphocyte-specific lck proximal promoter in transgenic mice[J].BMB Rep. 2008 Aug 31;41(8):575-80.