Difference between revisions of "Part:BBa K4032001"
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<partinfo>BBa_K4032001 short</partinfo>
 
<partinfo>BBa_K4032001 short</partinfo>
  
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This part contains sequences of fuse protein of amylase and RFP.
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NC_000913.3 malS alpha-amylase [Escherichia coli str. K-12 substr. MG1655] - Gene - NCBI (nih.gov)
  
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This enzyme hydrolyzes of (1-4)-alpha-D-glycosidic linkages in polysaccharides containing three or more (1-4)-alpha-linked D-glucose units.
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This part contains the gene of the amylase-RFP fusion protein.
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Red fluorescence of RFP is observed under the fluorescence microscope.
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This part expresses the fuse protein of amylase (from BBa_K523006) and RFP (from BBa_J04450). We removed the stop codon of amylase and the start codon of RFP.
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Usage and Biology
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This enzyme hydrolyzes of (1-4)-alpha-D-glycosidic linkages in polysaccharides containing three or more (1-4)-alpha-linked D-glucose units. See details NC_000913.3 (https://www.ncbi.nlm.nih.gov/gene/948088).
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In the case of this part,  red fluorescence of RFP is observed under the fluorescence microscope, but isn’t observed under natural light.
  
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This part expresses the fuse protein of amylase (from BBa_K523006 (https://parts.igem.org/Part:BBa_K523006) ) and RFP (from BBa_J04450 (https://parts.igem.org/Part:BBa_J04450) ). We removed the stop codon of amylase and the start codon of RFP and connected the ends.
  
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We also created BBa_K40320xx by adding RBS to this part.  
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The RBS is added to this part forming BBa_K4032xx.
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We also created BBa_K40321xx by adding lac promoter and RBS and double terminator to this part.  
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The lac promoter, the RBS and the double terminator are added to this part forming BBa_K4032xx.
  
  

Revision as of 07:54, 20 October 2021


No part name specified with partinfo tag.

NC_000913.3 malS alpha-amylase [Escherichia coli str. K-12 substr. MG1655] - Gene - NCBI (nih.gov)

This part contains the gene of the amylase-RFP fusion protein.

Usage and Biology This enzyme hydrolyzes of (1-4)-alpha-D-glycosidic linkages in polysaccharides containing three or more (1-4)-alpha-linked D-glucose units. See details NC_000913.3 (https://www.ncbi.nlm.nih.gov/gene/948088). In the case of this part, red fluorescence of RFP is observed under the fluorescence microscope, but isn’t observed under natural light.

This part expresses the fuse protein of amylase (from BBa_K523006 (https://parts.igem.org/Part:BBa_K523006) ) and RFP (from BBa_J04450 (https://parts.igem.org/Part:BBa_J04450) ). We removed the stop codon of amylase and the start codon of RFP and connected the ends.

The RBS is added to this part forming BBa_K4032xx. The lac promoter, the RBS and the double terminator are added to this part forming BBa_K4032xx.


Sequence and Features No part name specified with partinfo tag.