Difference between revisions of "Part:BBa K3889025"
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YqcG toxin is a part of type 2 toxin-antitoxin system namely YqcGF from <i>Bacillus subtilis</i> [1]. YqcF (<partinfo>BBa_K3889025</partinfo>) binds to YqcG in order to inhibit the toxin. YqcG is a DNase that can lead to DNA degradation finally causing death due to loss of genetic material. | YqcG toxin is a part of type 2 toxin-antitoxin system namely YqcGF from <i>Bacillus subtilis</i> [1]. YqcF (<partinfo>BBa_K3889025</partinfo>) binds to YqcG in order to inhibit the toxin. YqcG is a DNase that can lead to DNA degradation finally causing death due to loss of genetic material. | ||
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+ | This is a modified version specific of <partinfo>BBa_K3507002</partinfo> to our project's assembly. Xho1, HindIII and Bsa1 sites were mutated to make an assembly compatible part. | ||
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Revision as of 06:38, 20 October 2021
Bacillus subtilis yqcG toxin
YqcG toxin is a part of type 2 toxin-antitoxin system namely YqcGF from Bacillus subtilis [1]. YqcF (BBa_K3889025) binds to YqcG in order to inhibit the toxin. YqcG is a DNase that can lead to DNA degradation finally causing death due to loss of genetic material.
This is a modified version specific of BBa_K3507002 to our project's assembly. Xho1, HindIII and Bsa1 sites were mutated to make an assembly compatible part.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
1. Brantl, S., & Müller, P. (2019). Toxin–Antitoxin Systems in Bacillus subtilis. In Toxins (Vol. 11, Issue 5, p. 262). MDPI AG. https://doi.org/10.3390/toxins11050262