Difference between revisions of "Part:BBa M10224"
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<partinfo>BBa_M10224 short</partinfo> | <partinfo>BBa_M10224 short</partinfo> | ||
Composite part with an Arabinose promoter {Pbad}, ribosome binding site {rbs}, prepro periplasmic targetting sequence {prepro}, a streptavidin-binding tag {StrepTag}, a leucine zipper peptide {GS%-IILK}, circularly permutated ompX {CPompX} which functions as the carrier protein for display of AG4 on the cell surface of E.coli, and a terminator {dblTerm} | Composite part with an Arabinose promoter {Pbad}, ribosome binding site {rbs}, prepro periplasmic targetting sequence {prepro}, a streptavidin-binding tag {StrepTag}, a leucine zipper peptide {GS%-IILK}, circularly permutated ompX {CPompX} which functions as the carrier protein for display of AG4 on the cell surface of E.coli, and a terminator {dblTerm} | ||
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+ | [[Template:BioE140LSpr09-Streptavidin | Streptavidin binding assay template]]<br> | ||
+ | [[Template:BioE140LSpr09-Growth | Growth curve assay template]]<br> | ||
+ | [[Template:BioE140LSpr09-Autoaggregation | Autoaggregation assay template]]<br> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 04:47, 11 May 2009
{Pbad.rbs.prepro.StrepTag}{GS5-IILK}{CPompX!}{dblTerm}
Composite part with an Arabinose promoter {Pbad}, ribosome binding site {rbs}, prepro periplasmic targetting sequence {prepro}, a streptavidin-binding tag {StrepTag}, a leucine zipper peptide {GS%-IILK}, circularly permutated ompX {CPompX} which functions as the carrier protein for display of AG4 on the cell surface of E.coli, and a terminator {dblTerm}
Streptavidin binding assay template
Growth curve assay template
Autoaggregation assay template
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1784
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1249
Illegal PstI site found at 1784 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1784
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1784
Illegal AgeI site found at 1023 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1005