Difference between revisions of "Part:BBa M10218"
Jennbrophy (Talk | contribs) |
|||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_M10218 short</partinfo> | <partinfo>BBa_M10218 short</partinfo> | ||
Composite part with an Arabinose promoter {Pbad}, ribosome binding site {rbs}, prepro periplasmic targetting sequence {prepro}, a streptavidin-binding tag {StrepTag}, a leucine zipper peptide {AG4}, circularly permutated ompG {CPG_L6} which functions as the carrier protein for display of AG4 on the cell surface of E.coli, and a terminator {dblTerm} | Composite part with an Arabinose promoter {Pbad}, ribosome binding site {rbs}, prepro periplasmic targetting sequence {prepro}, a streptavidin-binding tag {StrepTag}, a leucine zipper peptide {AG4}, circularly permutated ompG {CPG_L6} which functions as the carrier protein for display of AG4 on the cell surface of E.coli, and a terminator {dblTerm} | ||
| + | |||
| + | [[Template:BioE140LSpr09-Streptavidin | Streptavidin binding assay template]]<br> | ||
| + | [[Template:BioE140LSpr09-Growth | Growth curve assay template]]<br> | ||
| + | [[Template:BioE140LSpr09-Autoaggregation | Autoaggregation assay template]]<br> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 04:46, 11 May 2009
{Pbad.rbs.prepro.StrepTag}{GS5-IILK}{CPG_L6!}{dblTerm}
Composite part with an Arabinose promoter {Pbad}, ribosome binding site {rbs}, prepro periplasmic targetting sequence {prepro}, a streptavidin-binding tag {StrepTag}, a leucine zipper peptide {AG4}, circularly permutated ompG {CPG_L6} which functions as the carrier protein for display of AG4 on the cell surface of E.coli, and a terminator {dblTerm}
Streptavidin binding assay template
Growth curve assay template
Autoaggregation assay template
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1249
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1023
Illegal AgeI site found at 2228 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1005