Difference between revisions of "Part:BBa K3726067:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
.
+
The part is a MoClo Lv.1 part, assembled in the acceptor Lv1 entry vector “BBa_K3228069” P1_pANs_SpecR, which is a self replicating shuttle vector.  
−
 
+
This part has been assembled following the marburg collection standard, then the transcriptional unit is flanked by the part“BBa_K3726108” 5CON1(H)_NS3(mod)-up (PCC 11801) that allows homologous recombination within the PCC 11801 Genome, and by the part  3'Con1 “BBa_K2560070” that will allow  the assembly of a Lv2 construct.
  
 +
This design will allow us to test which “tolerance and secretion ” variant has better efficiency by its own account as a replicative vector, and then assemble them as an integrative Lv2 Moclo construct. To know more about this, visit our webpage: https://2021.igem.org/Team:MADRID_UCM/Design
 +
.
  
 
===Source===
 
===Source===
  
−
.
+
This construct has been made by golden gate reaction.
  
 
===References===
 
===References===
 +
 +
"Using Transcriptomics To Improve Butanol Tolerance of Synechocystis sp. Strain PCC 6803 | Applied and Environmental Microbiology", Applied and Environmental Microbiology, 2021.

Latest revision as of 01:32, 20 October 2021


Lv.1 HspA - B


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 798
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 798
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2654
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 798
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 798
    Illegal NgoMIV site found at 724
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is a MoClo Lv.1 part, assembled in the acceptor Lv1 entry vector “BBa_K3228069” P1_pANs_SpecR, which is a self replicating shuttle vector. This part has been assembled following the marburg collection standard, then the transcriptional unit is flanked by the part“BBa_K3726108” 5CON1(H)_NS3(mod)-up (PCC 11801) that allows homologous recombination within the PCC 11801 Genome, and by the part 3'Con1 “BBa_K2560070” that will allow the assembly of a Lv2 construct.

This design will allow us to test which “tolerance and secretion ” variant has better efficiency by its own account as a replicative vector, and then assemble them as an integrative Lv2 Moclo construct. To know more about this, visit our webpage: https://2021.igem.org/Team:MADRID_UCM/Design .

Source

This construct has been made by golden gate reaction.

References

"Using Transcriptomics To Improve Butanol Tolerance of Synechocystis sp. Strain PCC 6803 | Applied and Environmental Microbiology", Applied and Environmental Microbiology, 2021.