Difference between revisions of "Part:BBa K3993012"

Revision as of 01:32, 20 October 2021

PTDH3-AsADC-TCYC1

Profile

Name: PTDH3-AsADC-TCYC1

Base Pairs: 2736bp

Origin: Saccharomyces cerevisiae, E. coli, synthesis

Properties: Arginine metabolism and polyamine biosynthesis chemical reactions

Usage and Biology

Kl tropane alkaloids (TAs) refers to a kind of alkaloids containing the tropane alkyl skeleton formed by the combination of pyrrole ring and piperidine ring in structure. It is a natural product of plant and has a long history and important medicinal value. tropane alkaloids have great market demand and often appear in global shortages. A method that can produce Tas in scale is expected. Using synthetic biology to create a microbial cell factory to produce TAs is a highly potential strategy.

The Tropane alkaloid (TAs) is obtained by a series of chemical reactions through the formation of Putrescine (1, 4-butylenediamine, Putrescine) from Arginine. Putrescine is an essential polyamine for ribosomal biogenesis and mRNA translation, but is regulated by polyamines and remains at low concentrations during normal cell growth. In this study, by overexpressing the natural genes involved in arginine metabolism and polyamine biosynthesis, the regulatory mechanism of polyamine biosynthesis is adjusted, so as to engineer the production of excessive putrescine strains.

Figure1. Principle diagram of TAs..

Construct design

The Tropine part of Tropane alkaloids (TAs) is obtained from arginine to putrescine (1,4-butanediamine, putrescine), and then through a series of chemical reactions. In this project, natural genes involved in arginine metabolism and polyamine biosynthesis was designed to overexpress in yeast. The engineer strains that produced excess putrescine. (Figure 2).

Figure 2. DNA sequence map of plasmid PTDH3-AsADC-TCYC1..

Sequence and Features