Difference between revisions of "Part:BBa K3792000"

Revision as of 01:23, 20 October 2021

NodI

Protein possibly involved in transporting chitin across the cell membrane. Expressed with nodC and nodJ by the 2021 FSU team.

To test if the cells were secreting chitin, The FSU 2021 team used the Chitosan Assay Kit (XAN-5126) available from Cell Biolabs. The manual for this kit contains the protocols for converting chitin to chitosan, along with a protocol for detecting chitosan.

Chitin from the cells was converted into chitosan by reacting with 12.5M NaOH at 95°C for 24 hours. The solution was then neutralized with acetic acid. The assay was then performed on the supernatant of the cell culture.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 150

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3792000 short</partinfo>
-Protein possibly involved in transporting chitin across the cell membrane. Expressed with nodC and nodJ.
+Protein possibly involved in transporting chitin across the cell membrane. Expressed with nodC and nodJ by the 2021 FSU team.
+To test if the cells were secreting chitin, The FSU 2021 team used the Chitosan Assay Kit (XAN-5126) available from Cell Biolabs. The manual for this kit contains the protocols for converting chitin to chitosan, along with a protocol for detecting chitosan.
+Chitin from the cells was converted into chitosan by reacting with 12.5M NaOH at 95°C for 24 hours. The solution was then neutralized with acetic acid. The assay was then performed on the supernatant of the cell culture.
 <!-- Add more about the biology of this part here