Difference between revisions of "Part:BBa K3792009"

Latest revision as of 01:04, 20 October 2021

NodC Generator I

The NodC Control Generator with Weak Promoter produces the NodC protein. The NodC protein produces the carbohydrate, chitin.The NodC Generator with Weak Promoter does not produce a lot of the NodC protein. The NodC protein produces chitin.

This part consists of 821 base-pair length. It includes a promoter, 1 ribosome binding site, 1 protein-coding sequence, and a terminator. We used promoter K2832184, a weak promoter from the BIOFAB collection. In addition, we also used a protein-coding sequence from Chitin synthase from Rhiozobium leguminosarum.

This generator was assembled into the pSB1K3 plasmid, and then transformed into E.Coli NEB 5-Alpha. To test if the cells were secreting chitin, The FSU 2021 team used the Chitosan Assay Kit (XAN-5126) available from Cell Biolabs. The manual for this kit contains the protocols for converting chitin to chitosan, along with a protocol for detecting chitosan.

Chitin from the cells was converted into chitosan by reacting with 12.5M NaOH at 95°C for 24 hours. The solution was then neutralized with acetic acid. The assay was then performed on the supernatant of the cell culture.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 492
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3792009 short</partinfo>
-The NodC Control Generator with Weak Promoter produces the NodC protein. The NodC protein produces the protein, chitin.
+The NodC Control Generator with Weak Promoter produces the NodC protein. The NodC protein produces the carbohydrate, chitin.The NodC Generator with Weak Promoter does not produce a lot of the NodC protein. The NodC protein produces chitin.
+This part consists of 821 base-pair length. It includes a promoter, 1 ribosome binding site, 1 protein-coding sequence, and a terminator. We used promoter K2832184, a weak promoter from the BIOFAB collection. In addition, we also used a protein-coding sequence from Chitin synthase from Rhiozobium leguminosarum.
+This generator was assembled into the pSB1K3 plasmid, and then transformed into E.Coli NEB 5-Alpha. To test if the cells were secreting chitin, The FSU 2021 team used the Chitosan Assay Kit (XAN-5126) available from Cell Biolabs. The manual for this kit contains the protocols for converting chitin to chitosan, along with a protocol for detecting chitosan.
+Chitin from the cells was converted into chitosan by reacting with 12.5M NaOH at 95°C for 24 hours. The solution was then neutralized with acetic acid. The assay was then performed on the supernatant of the cell culture.
 <!-- Add more about the biology of this part here