Difference between revisions of "Part:BBa K3833000"

Revision as of 00:51, 20 October 2021

Streptococcal Collagen-Like Protein

Streptococcal Collagen-Like Protein (Scl2) was originally discovered in gram-positive S. pyogenes. It is a prokaryotic protein that can be used to mimic human collagen and has been shown to form stable triple helices[1].

Scl2 is easily expressed in E. coli and lacks the requirement for post-translational modifications; this makes it a useful alternative to recombinant human collagen expressed in eukaryotic organisms[1]. For these reasons, we chose Scl2 as the backbone for Collatrix — a collagen-mimetic gradient hydrogel for bone-soft tissue interfaces.

Scl2 can be modified to specific locations with common mammalian cell binding sites, such as fibronectin and integrin [2]. After is it produced, it can be cross-linked both chemically (with the use of glutaraldehyde) and with UV light in order to create hydrogels to serve as scaffolds for cell growth.

[1] An, B., Kaplan, D. L., & Brodsky, B. (2014). Engineered recombinant bacterial collagen as an alternative collagen-based biomaterial for tissue engineering. Frontiers in Chemistry, 2, 40. https://doi.org/10.3389/fchem.2014.00040

[2] Parenteau-Bareil, R., Gauvin, R., & Berthod, F. (2010). Collagen-Based Biomaterials for Tissue Engineering Applications. Materials, 3(3), 1863–1887. https://doi.org/10.3390/ma3031863

Sequence and Features