Difference between revisions of "Part:BBa K3993002"
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<partinfo>BBa_K3993002 short</partinfo> | <partinfo>BBa_K3993002 short</partinfo> | ||
| − | AsADC | + | === Profile === |
| + | ====Name: AsADC==== | ||
| + | ====Base Pairs: 1821bp==== | ||
| + | ====Origin: Saccharomyces cerevisiae, synthesis==== | ||
| + | ====Properties: codon optimized SPE1==== | ||
| + | |||
| + | === Usage and Biology === | ||
| + | This protein is involved in step 1 of the subpathway that synthesizes agmatine from L-arginine. This subpathway is part of the pathway agmatine biosynthesis, which is itself part of Amine and polyamine biosynthesis. | ||
| + | |||
| + | This protein is involved in step 1 of the subpathway that synthesizes agmatine from L-arginine. This subpathway is part of the pathway agmatine biosynthesis, which is itself part of Amine and polyamine biosynthesis. | ||
| + | |||
| + | [[File:T--SHSID--BBa K3993000 Figure1.png|500px|thumb|center|Figure1. Principle diagram of TAs..]] | ||
| + | |||
| + | === Experimental approach === | ||
| + | 1. Fragments PCR products Electrophoresis | ||
| + | |||
| + | [[File:T--SHSID--BBa K3993012-Figure2.png|500px|thumb|center|Figure 2. Gel electrophoresis of amplified fragments..]] | ||
| + | |||
| + | Lane 1 is target gene AsADC | ||
| + | |||
| + | === proof of function === | ||
| + | Modeling for predicting the performance of our engineered bacteria to produce tropine | ||
| + | |||
| + | Firstly, we get the polynomial linear regression-2 shown in figure 3. (Data from the published articles, according to references3/4 ) | ||
| + | |||
| + | [[File:T--SHSID--BBa K3993012-Figure3.png|500px|thumb|center|Figure 3..]] | ||
| + | |||
| + | The R-squared reaches 0.9855, which can be used to predict the performance of our engineered bacteria to produce tropine. | ||
| + | Substituting the time and the OD600 value we tested in the laboratory into the model to get figures 4. | ||
| + | |||
| + | [[File:T--SHSID--BBa K3993012-Figure4.png|500px|thumb|center|Figure 4..]] | ||
| + | |||
| + | The results show that when the OD600 of our engineered bacteria reaches a certain value, the output of tropine will increase sharply, indicating that our engineered bacteria have great industrial application prospects. | ||
| + | |||
| + | === References === | ||
| + | ====1. Srinivasan, P., Smolke, C.D. Biosynthesis of medicinal tropane alkaloids in yeast. Nature 585, 614–619 (2020).==== | ||
| + | ====2. Srinivasan, P., Smolke, C.D. Engineering a microbial biosynthesis platform for de novo production of tropane alkaloids. Nat Commun 10, 3634 (2019).==== | ||
| + | ====3. Prashanth Srinivasan & Christina D. Smolke. Biosynthesis of medicinal tropane alkaloids in yeast.Nature | Vol 585 | 24 September 2020 | 614-619==== | ||
| + | ====4. Prashanth Srinivasan & Christina D. Smolke. Engineering a microbial biosynthesis platform for de novo production of tropane alkaloids.NATURE COMMUNICATIONS | https://doi.org/10.1038/s41467-019-11588-w==== | ||
| + | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | |||
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 00:49, 20 October 2021
AsADC
Profile
Name: AsADC
Base Pairs: 1821bp
Origin: Saccharomyces cerevisiae, synthesis
Properties: codon optimized SPE1
Usage and Biology
This protein is involved in step 1 of the subpathway that synthesizes agmatine from L-arginine. This subpathway is part of the pathway agmatine biosynthesis, which is itself part of Amine and polyamine biosynthesis.
This protein is involved in step 1 of the subpathway that synthesizes agmatine from L-arginine. This subpathway is part of the pathway agmatine biosynthesis, which is itself part of Amine and polyamine biosynthesis.
Experimental approach
1. Fragments PCR products Electrophoresis
Lane 1 is target gene AsADC
proof of function
Modeling for predicting the performance of our engineered bacteria to produce tropine
Firstly, we get the polynomial linear regression-2 shown in figure 3. (Data from the published articles, according to references3/4 )
The R-squared reaches 0.9855, which can be used to predict the performance of our engineered bacteria to produce tropine. Substituting the time and the OD600 value we tested in the laboratory into the model to get figures 4.
The results show that when the OD600 of our engineered bacteria reaches a certain value, the output of tropine will increase sharply, indicating that our engineered bacteria have great industrial application prospects.
References
1. Srinivasan, P., Smolke, C.D. Biosynthesis of medicinal tropane alkaloids in yeast. Nature 585, 614–619 (2020).
2. Srinivasan, P., Smolke, C.D. Engineering a microbial biosynthesis platform for de novo production of tropane alkaloids. Nat Commun 10, 3634 (2019).
3. Prashanth Srinivasan & Christina D. Smolke. Biosynthesis of medicinal tropane alkaloids in yeast.Nature | Vol 585 | 24 September 2020 | 614-619
4. Prashanth Srinivasan & Christina D. Smolke. Engineering a microbial biosynthesis platform for de novo production of tropane alkaloids.NATURE COMMUNICATIONS | https://doi.org/10.1038/s41467-019-11588-w
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 689
Illegal BglII site found at 910 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]