Difference between revisions of "Part:BBa K3792005"

 
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<partinfo>BBa_K3792005 short</partinfo>
 
<partinfo>BBa_K3792005 short</partinfo>
  
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The apFAB51 test device does not produce a lot of the mRFP1 protein. The protein, mRFP1, is a red fluorescent protein.  
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apFAB51 is a weak promoter taken from the BIOFAB collection. This device uses apFAB51 to express mRFP1, a red fluorescent protein. The apFAB51 test device consists of apFAB51, an RBS, the coding sequence for mRFP1, and a terminator.
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This test device and two others with BIOFAB promoters of different strengths were each assembled into the pSB1K3 plasmid and then transformed into E. coli NEB 5 alpha.
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The fluorescence of the cells was measured using flow cytometry. By comparing the relative fluorescence, we can determine which promoters caused higher levels of expression of mRFP1.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3792005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3792005 SequenceAndFeatures</partinfo>
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K3792005 parameters</partinfo>
 
<partinfo>BBa_K3792005 parameters</partinfo>
 
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Revision as of 00:42, 20 October 2021

BIOFAB apFAB51 Measurement Device

apFAB51 is a weak promoter taken from the BIOFAB collection. This device uses apFAB51 to express mRFP1, a red fluorescent protein. The apFAB51 test device consists of apFAB51, an RBS, the coding sequence for mRFP1, and a terminator.

This test device and two others with BIOFAB promoters of different strengths were each assembled into the pSB1K3 plasmid and then transformed into E. coli NEB 5 alpha.

The fluorescence of the cells was measured using flow cytometry. By comparing the relative fluorescence, we can determine which promoters caused higher levels of expression of mRFP1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 614
    Illegal AgeI site found at 726
  • 1000
    COMPATIBLE WITH RFC[1000]