Difference between revisions of "Part:BBa K3993001"
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This protein is involved in step 1 of the subpathway that synthesizes putrescine from agmatine. This subpathway is part of the pathway putrescine biosynthesis via agmatine pathway, which is itself part of Amine and polyamine biosynthesis. The expression of AUH activity is antagonistically regulated by cyclic AMP and agmatine. In the presence of the cAMP receptor protein, cAMP represses the expression of AUH, while agmatine induces it. | This protein is involved in step 1 of the subpathway that synthesizes putrescine from agmatine. This subpathway is part of the pathway putrescine biosynthesis via agmatine pathway, which is itself part of Amine and polyamine biosynthesis. The expression of AUH activity is antagonistically regulated by cyclic AMP and agmatine. In the presence of the cAMP receptor protein, cAMP represses the expression of AUH, while agmatine induces it. | ||
| − | [[File:T--SHSID--BBa K3993000 Figure1.png|500px|thumb|center|Figure1. Principle diagram of TAs..] | + | [[File:T--SHSID--BBa K3993000 Figure1.png|500px|thumb|center|Figure1. Principle diagram of TAs..]] |
=== Experimental approach === | === Experimental approach === | ||
1. Fragments PCR products Electrophoresis | 1. Fragments PCR products Electrophoresis | ||
| − | [[File:T--SHSID--BBa K3993001 Figure2.png|500px|thumb|center|Figure 2. Gel electrophoresis of amplified fragments..] | + | [[File:T--SHSID--BBa K3993001 Figure2.png|500px|thumb|center|Figure 2. Gel electrophoresis of amplified fragments..]] |
Lane 1 is target gene SpeB | Lane 1 is target gene SpeB | ||
Latest revision as of 00:41, 20 October 2021
SPEB
Profile
Name: SPEB
Base Pairs: 921bp
Origin: E. coli, genome
Properties: Catalyzes the formation of putrescine from agmatine.
Usage and Biology
This protein is involved in step 1 of the subpathway that synthesizes putrescine from agmatine. This subpathway is part of the pathway putrescine biosynthesis via agmatine pathway, which is itself part of Amine and polyamine biosynthesis. The expression of AUH activity is antagonistically regulated by cyclic AMP and agmatine. In the presence of the cAMP receptor protein, cAMP represses the expression of AUH, while agmatine induces it.
Experimental approach
1. Fragments PCR products Electrophoresis
Lane 1 is target gene SpeB
References
1. Srinivasan, P., Smolke, C.D. Biosynthesis of medicinal tropane alkaloids in yeast. Nature 585, 614–619 (2020).
2. Srinivasan, P., Smolke, C.D. Engineering a microbial biosynthesis platform for de novo production of tropane alkaloids. Nat Commun 10, 3634 (2019).
3. Prashanth Srinivasan & Christina D. Smolke. Biosynthesis of medicinal tropane alkaloids in yeast.Nature | Vol 585 | 24 September 2020 | 614-619
4. Prashanth Srinivasan & Christina D. Smolke. Engineering a microbial biosynthesis platform for de novo production of tropane alkaloids.NATURE COMMUNICATIONS | https://doi.org/10.1038/s41467-019-11588-w
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 789
Illegal BamHI site found at 702 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 724
- 1000COMPATIBLE WITH RFC[1000]