Difference between revisions of "Part:BBa K3993001"

 
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<partinfo>BBa_K3993001 short</partinfo>
 
<partinfo>BBa_K3993001 short</partinfo>
  
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SPEB
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===  Profile ===
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====Name: SPEB====
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====Base Pairs: 921bp====
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====Origin: E. coli, genome====
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====Properties: Catalyzes the formation of putrescine from agmatine.====
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=== Usage and Biology ===
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This protein is involved in step 1 of the subpathway that synthesizes putrescine from agmatine. This subpathway is part of the pathway putrescine biosynthesis via agmatine pathway, which is itself part of Amine and polyamine biosynthesis. The expression of AUH activity is antagonistically regulated by cyclic AMP and agmatine. In the presence of the cAMP receptor protein, cAMP represses the expression of AUH, while agmatine induces it.
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[[File:T--SHSID--BBa K3993000 Figure1.png|500px|thumb|center|Figure1. Principle diagram of TAs..]
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=== Experimental approach ===
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1. Fragments PCR products Electrophoresis
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[[File:T--SHSID--BBa K3993001 Figure2.png|500px|thumb|center|Figure 2. Gel electrophoresis of amplified fragments..]
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Lane 1 is target gene SpeB
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=== References ===
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====1. Srinivasan, P., Smolke, C.D. Biosynthesis of medicinal tropane alkaloids in yeast. Nature 585, 614–619 (2020).====
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====2. Srinivasan, P., Smolke, C.D. Engineering a microbial biosynthesis platform for de novo production of tropane alkaloids. Nat Commun 10, 3634 (2019). ====
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====3.  Prashanth Srinivasan & Christina D. Smolke. Biosynthesis of medicinal tropane alkaloids in yeast.Nature | Vol 585 | 24 September 2020 | 614-619 ====
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====4. Prashanth Srinivasan & Christina D. Smolke. Engineering a microbial biosynthesis platform for de novo production of tropane alkaloids.NATURE COMMUNICATIONS | https://doi.org/10.1038/s41467-019-11588-w====
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 00:39, 20 October 2021


SPEB

Profile

Name: SPEB

Base Pairs: 921bp

Origin: E. coli, genome

Properties: Catalyzes the formation of putrescine from agmatine.

Usage and Biology

This protein is involved in step 1 of the subpathway that synthesizes putrescine from agmatine. This subpathway is part of the pathway putrescine biosynthesis via agmatine pathway, which is itself part of Amine and polyamine biosynthesis. The expression of AUH activity is antagonistically regulated by cyclic AMP and agmatine. In the presence of the cAMP receptor protein, cAMP represses the expression of AUH, while agmatine induces it.

[[File:T--SHSID--BBa K3993000 Figure1.png|500px|thumb|center|Figure1. Principle diagram of TAs..]

Experimental approach

1. Fragments PCR products Electrophoresis

[[File:T--SHSID--BBa K3993001 Figure2.png|500px|thumb|center|Figure 2. Gel electrophoresis of amplified fragments..]

Lane 1 is target gene SpeB

References

1. Srinivasan, P., Smolke, C.D. Biosynthesis of medicinal tropane alkaloids in yeast. Nature 585, 614–619 (2020).

2. Srinivasan, P., Smolke, C.D. Engineering a microbial biosynthesis platform for de novo production of tropane alkaloids. Nat Commun 10, 3634 (2019).

3. Prashanth Srinivasan & Christina D. Smolke. Biosynthesis of medicinal tropane alkaloids in yeast.Nature | Vol 585 | 24 September 2020 | 614-619

4. Prashanth Srinivasan & Christina D. Smolke. Engineering a microbial biosynthesis platform for de novo production of tropane alkaloids.NATURE COMMUNICATIONS | https://doi.org/10.1038/s41467-019-11588-w

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 789
    Illegal BamHI site found at 702
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 724
  • 1000
    COMPATIBLE WITH RFC[1000]